Electrophoresis of Lactic Dehydrogenase in Borate-buffered Starch-gel
نویسنده
چکیده
It has been suggested-:" that examination of the serum lactic dehydrogenase (LDH) isoenzyme pattern may be helpful in identifying tissues affected by disease. Cellular damage to tissues such as myocardium or liver results in characteristic abnormalities in the relative proportions of the 5 LDH isoenzymes demonstrable in serum by electrophoresis in barbituratebuffered agar2•4, starch-grain! and starch-gel" media. Unfortunately, as might be predicted from tissue LDH isoenzyme patterns'', serum isoenzyme patterns are not entirely tissue-specifics, particularly when there is a possibility of more than one tissue contributing to the serum LDH. The ability of electrophoresis in borate-buffered starch-gel! to detect many more serum protein fractions than other electrophoretic methods suggested that this procedure might also resolve serum LDH into isoenzyme patterns ofgreater tissue specificity. Some support for this was found in the results of Markert and M011er6, who applied this method to a study ofLDH isoenzymes in animal tissues.
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